A rat model of septic shock using live <i>Escherichia coli</i> and response to Noradrenaline

Objectives: Popular animal models of septic shock involve injections endotoxin (bacterial lipopolysaccharide). Other methods that induce sepsis are often time-consuming and require long-term monitoring facilities. Further, individual using different bacterial strains can deepen our understanding pathophysiology. Hence, objective was to develop an acute functional Wistar rat model live Escherichia coli then administer Noradrenaline, a known sympathomimetic drug, study if the response is along expected lines. Materials Methods: After random allocation one three groups (Group 1 – E. alone, n=7; Group 2 followed by n = 7 3 control ( 4), which received saline injections), rats were anesthetised intra-arterial pressure recorded from carotid artery catheter. Live suspended in normal (5 Mcfarland concentration; dose 650 uL/100 g body weight) injected through tail vein sepsis. When mean arterial dropped 50% its value before injection, Noradrenaline 2. Results: The average time (t1, 14) for set about 1.94 ± 0.97 h. Six out seven 1) died within 60 min without intervention. addition after hypotension prolonged death significantly 170 min. Conclusion: described acute, stable, various aspects shock. Administration animal’s life as expected. Future studies other common agents encountered clinics be undertaken similarly.